mouse vegf elisa kit  (R&D Systems)

Journal: PLOS One

Article Title: Targeted transferosomal delivery of cetirizine: A new approach to alopecia management

doi: 10.1371/journal.pone.0347648

Figure Lengend Snippet: Insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) mRNA and protein expression levels in different groups.

Article Snippet: IGF-1 and VEGF protein levels in hair bulbs were measured using a Mouse & Rat IGF-I/IGF-1 ELISA Kit (Quantikine; R&D systems, Minneapolis, MN, USA) and a Mouse VEGF ELISA Kit (Quantikine; R&D systems), respectively, according to the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: Journal of Functional Biomaterials

Article Title: A Borophosphate Glass Doped with Cobalt Oxide Improves Skeletal Muscle Structure and Function in Myopathic Mice

doi: 10.3390/jfb17030155

Figure Lengend Snippet: TRIM enhances vascularity. ( A , B ) Representative images of TA cross-sections in WT and D2. mdx mice at 14 dpt. Laminin + immunostaining (white) identified basal laminae surrounding myofibers; CD31 + immunostaining (green) identifies endothelial cells as a measure of vascularity. Laminin intensity in the merged images was reduced to enhance visualization of CD31 + staining. Yellow arrows identify CD31 + staining in merged image. ( C ) Summary values ( n = 4–5/group) for area occupied by ECs were calculated as: total EC area/number (#) of fibers. ( D ) Summary values ( n = 4–5/group) for EC-to-myofiber ratio were calculated as: # of EC puncta/# of myofibers. Summary values presented for 14 dpt. Angiogenic factors are increased with TRIM. ( E ) VEGF concentration in whole-muscle TA homogenates ( n = 5/group) probed for by ELISA. Summary values are means ± SEM; comparisons made vs. WT and vehicle controls by 2-Way ANOVA, p < 0.05 = significant. Scale bars = 100 μm.

Article Snippet: Samples of muscle supernatant (50 μL; referred to above) were analyzed to determine VEGFA protein concentration using a Quantikine ELISA (Cat.# MMV00, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Immunostaining, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Functional Biomaterials

Article Title: A Borophosphate Glass Doped with Cobalt Oxide Improves Skeletal Muscle Structure and Function in Myopathic Mice

doi: 10.3390/jfb17030155

Figure Lengend Snippet: TRIM enhances vascularity. Representative images of TA cross-sections in D2. mdx Saline (Top) and D2. mdx TRIM (Bottom) mice. ( A ) A 70 dpt and ( E ) 140 dpt. Laminin+ immunostaining (white) identified basal laminae surrounding myofibers; CD31 + immunostaining (green) identifies endothelial cells as a measure of vascularity. Laminin intensity in the merged images was reduced to enhance visualization of CD31 + staining. Yellow arrows identify CD31 + staining in merged image. Summary values ( n = 7–8/group) for area occupied by ECs were calculated as: total EC area/number (#) of myofibers. Summary values presented for ( B ) 70 dpt and ( F ) 140 dpt. Summary values ( n = 7–8/group) for EC-to-myofiber ratio were calculated as: # of EC puncta/# of myofibers, presented for ( C ) 70 dpt and ( G ) 140 dpt. Angiogenic factors are increased with TRIM. VEGF concentration in whole muscle TA homogenates probed for by ELISA at ( D ) 70 dpt and ( H ) 140 dpt. ( n = 7–8/group); summary values are means ± SEM. Comparisons made vs. vehicle controls by two-tailed Student’s t -test; p < 0.05 = significant. Scale bars = 100 μm.

Article Snippet: Samples of muscle supernatant (50 μL; referred to above) were analyzed to determine VEGFA protein concentration using a Quantikine ELISA (Cat.# MMV00, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Saline, Immunostaining, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Molecular Therapy. Nucleic Acids

Article Title: Improving angiogenesis ameliorates the efficacy of ASO-based exon skipping for the treatment of Duchenne muscular dystrophy

doi: 10.1016/j.omtn.2026.102834

Figure Lengend Snippet: Angiogenesis improvement after mLVRF systemic injections (A) Timeline of the injection protocol. Groups of 7- to 10-week-old male mdx mice were injected intravenously with a single dose AAV9 encoding the mLVRF isoform or an AAV9 scramble as control (3E+13 vg/kg) ( n = 4–5 mice per group). Four weeks after this pre-treatment, mice were injected intravenously with ASO (50 mg/kg/week) or PBS during 12 weeks. Age-matched C57BL/10 wild-type mice were used as controls (WT). One week after the last injection, tissues were collected as described in the section. (B) Quantification of the expression of mLVRF performed by qPCR on gastrocnemius (GAS) total RNA. (C) VEGF concentration measured by ELISA on GAS protein lysates (∗ p < 0.05; ∗∗∗∗ p < 0.0001, analyzed by one-way ANOVA). (D) CD31 expression quantified by qPCR on total RNA extracted from GAS muscles (∗ p < 0.05, ∗∗∗ p < 0.001; analyzed by one-way ANOVA). (E) CD31 immunostaining of TA muscles from WT and scramble-, mLVRF-, ASO-, and mLVRF+ASO-treated mice. Scale bars, 50 μm. (F) Distribution of the proportion of fibers as a function of the number of associated blood vessels. (G) Quantification of the mean number of blood vessels per myofiber (∗ p < 0.05, ∗∗∗∗ p < 0.0001; analyzed by one-way ANOVA). (H and I) Proportion of fibers with no associated blood vessels (H) or associated with 5 vessels (I). (∗ p < 0.05, ∗∗ p < 0.01; analyzed by one-way ANOVA). Data regarding blood vessels were obtained from CD31 immunostaining. (J) Quantification of the VEGFR3 expression by qPCR on GAS RNA (∗∗ p < 0.01, analyzed by one-way ANOVA). Results are expressed as the mean ± SEM ( n = 4–5 per group).

Article Snippet: Tissue VEGF protein levels were quantified using the Quantikine Mouse VEGF ELISA kit (MMV00, R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions.

Techniques: Injection, Control, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Muscles, Immunostaining